Confocal Microscope
Awarded
The competition has ended and a winner has been selected.
- Deadline
- Feb 1,Expired
- Award
- Readiness for integration with automated microsampling in multiwell plate 13% and 4 more
Brief
Universiteit Leiden has entered into an agreement with Nikon Europe BV for the delivery of one advanced confocal microscope to be located at the Leiden Cell Observatory (LCO) in the Netherlands. The contract has a duration of 12 months and a total value of 495,868 EUR. The microscope is intended for image-guided single-cell sampling and isolation using confocal imaging and lifetime fluorescence, supporting high-throughput phenotypic screening in 3D live advanced cell systems and multi-omics approaches. Specific requirements included hardware capabilities such as label-free, lifetime, time-lapse, and automated high-content confocal microscopy with high reproducibility in Z focus. Software requirements included communication with third-party micro-sampling robotic software, smart microscopy functionality for automated detection of single-cell events, and FAIR compliance for imaging data, including compatibility with the OMERO database and OME-ZARR standards. The microscope also needed to have state-of-the-art reliability and comply with Dutch work environment safety regulations. Key quality criteria for the award included high-throughput imaging instruments (e.g., automatic water dispenser, 100 fps imaging speed, automatic correction collar, motorized fast piezo Z focus), readiness for integration with automated microsampling (e.g., high-precision motorized stage with 0.1μm resolution, willingness to modify software for microsampler integration, real-time imaging feedback), data generation and FAIR data management (e.g., OME.zarr generation, OMERO server interfacing), and service and warranty requirements (e.g., on-site training, free software updates, extended warranty options).
Laboratorie- og analyseutstyr
Result
- Nikon Europe B.V.Winner
- 1Number of bids
- €495,868Contract value
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Award Criteria
Description
Price of the microscope
Source
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Description
High-precision motorized stage with sub-micron accuracy in XYZ axes for exact positioning of the sampling needle XYZ axial designated resolution: 0.1μm. Willingness to modify/open software to accommodate the microsampler. Compatible with integration of needle control software with the confocal microscope control software for coordinated movements. Compatible with real-time imaging feedback to ensure correct needle placement during sampling.
Source
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Description
Integrated image analysis software capable of identifying and tracking intracellular structures or specific cell phenotypes (including transmission light, fluorescence and lifetime). Real-time feedback mechanisms using smart microscopy for all channels (including transmission light, fluorescence and lifetime). Capability for automated workflows and scripting for custom experiments.
Source
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Description
The instrument should be equipped with an automatic water dispenser system for keeping the immersion liquid on the objectives. Imaging speed at full FOV 100 fps. Automatic correction collar that can be adjusted automatically for best optical performance via software without user’s manual input. Motorized fast piezo Z focus for 3D imaging.
Source
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Description
Comprehensive user training must be provided on-site at the time of installation. This should include hands-on training for operating the microscope, basic troubleshooting, and maintenance procedures. Detailed user manuals, maintenance guides, and troubleshooting documentation must be provided in both printed and electronic formats. The vendor must provide all software updates and upgrades free of charge. Updates should include new features, security patches, and performance improvements. The vendor should offer options for extending the warranty and service contracts beyond the initial warranty period. Details of extended warranty plans, including costs and coverage, should be provided.
Source
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Description
Software should generate OME.zarr, the Next Generation File Format (NGFF) for bioimaging data. Software capable of directly interfacing with the OMERO server for data storage, annotation, and sharing. Software should allow for the automatic upload of imaging data and metadata to the OMERO server without requiring manual intervention.
Source
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From the notice
The Leiden Academic Centre for Drug Research (LACDR) (part of the Faculty of Science of Leiden University) is seeking tenders for the procurement of one advanced microscope capable of performing image-guided single-cell sampling and isolation using confocal imaging and lifetime fluorescence. The microscope will be used for the Exposome Scan Dutch infrastructure and located at the Leiden Cell Observatory (LCO). With this microscope we aim to develop a microscopy-based infrastructure that enables both i) high-throughput phenotypic screening in 3D live advanced cell systems and ii) image-guided single-cell and intracellular component sampling. This infrastructure will serve scientists interested in screening relevant compound libraries in a wide variety of advanced fluorescent reporter cell systems (e.g., human stem cells cultured in 2D and differentiated in 3D microtissues such as liver or kidney organoids) and obtaining a mechanistic understanding of specific imaged phenotypes using a multi-omics approach. The microscope hardware must provide the following functionality: 1) Hardware: Label-free (brightfield or transmission), and lifetime microscopy. Time-lapse microscopy (incubator for a controlled environment). Confocal microscopy for 2D and 3D reporter cell systems cultured in multi-well plates. Automated high-content microscopy (automated stage with high X, Y, Z accuracy for both screening and automated micro sampling). High reproducibility in Z focus needed for time-lapse 2D/3D microscopy (perfect focus/autofocus). 2) Software: Software must be able to communicate with third-party software controlling the micro-sampling robotic. Smart microscopy functionality that allows the automated detection of single-cell events such as: Recognition of intracellular organelles or parts of the cytoplasm. Recognition of a cell next to a target cell. Recognition of cells with different morphologies. Recognition of cells or intracellular components with different lifetime signals. Recognition of specific cell regions based on different fluorescent lifetime signals. Single-cell cloning from cells displaying a unique phenotype. 4) FAIR Compliance: Imaging data must comply with the FAIR principles, including compatibility with the OMERO database and preferentially generating OME-ZARR standards. The microscope must have state-of-the-art reliability and be compatible with Dutch work environment safety regulations (ARBO).
Contact
Govert Schipperheijninkoop@ufb.leidenuniv.nl- ResultType
- Agglomeratie Leiden en BollenstreekLocation
- 38510000Main codeMicroscopes
- 38634000Optical microscopes