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    Purchase of electron microscopes for DANCEM

    Aarhus Universitet

    Purchase of electron microscopes for DANCEM

    1. Questions deadline

      Jan 13, 11:00 AMJanuary 13, 2026 11:00 AM

    2. Tender submission deadline

      Jan 20, 11:00 AMJanuary 20, 2026 11:00 AM

    3. Tender validity period

      Apr 20, 10:00 AMApril 20, 2026 10:00 AM

    4. Result notice published

      Sep 30, 6:21 AMSeptember 30, 2026 6:21 AM

    Brief

    Denmark: Tenders/Aarhus Universitet/Purchase of electron microscopes for DANCEM
    Award
    Procedure
    Without prior noticeTwo stages
    Agreement
    Buying goods

    Brief

    Aarhus Universitet has entered into an agreement with FEI Europe BV. Branch Denmark for the delivery of six electron microscopes for the Danish National Cryogenic Electron Microscopy Infrastructure (DANCEM). The microscopes, including Krios 5 300 kV cryo-TEMs, Talos 12 120 kV TEMs, an Arctis cryo-plasma-FIB, and a Hydra Bio multi-ion cryo-plasma-FIB, will be delivered to Aarhus University and Copenhagen University in Østjylland and Byen København. The purchase aims to support research in various areas such as neuroscience, genome editing, infection biology, cancer research, and drug discovery, with a particular focus on establishing new cryogenic electron tomography research workflows. Specific requirements included the ability to transfer lamellae under vacuum between instruments, use the same autogrid format and cassette as existing instruments, and utilize the same Thermo Fisher Scientific acquisition, automation, and correlative software environment. Additionally, the AU node required a cryo-PFIB with four selectable ion species for thick samples and tissues, and each node needed a 120 kV screening TEM running the same software as the Krios 5.

    Laboratorie-, optisk- og presisjonsutstyr

    Result

    TED
    • FEI Europe BV. Branch Denmark
      Winner
    • Number of bids
      0
    • Contract value
      DKK 108,000,000

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    • Description

      The requirements come from the scientific objectives for DANCEM that aims at making the infrastructure available for a range of research areas including neuroscience, genome editing, infection biology, cancer research, and drug discovery employing workflows in single particle analysis, microcrystal electron diffraction and cryogenic electron tomography. DANCEM will in particular focus on establishing new cryogenic electron tomography research workflows. The DANCEM user base covers research in cells, tissues, microbial communities, plant materials and materials science that need the essential requirements described below. The DANCEM consortium already operates TFS instruments: Titan Krios G2, Glacios and Hydra Bio at UCPH; Titan Krios G3i, Titan Krios G1 and Aquilos 2 at AU. • R1 Lamellae must be transferred from the cryo-PFIB to the cryo-TEM under vacuum, autoloader to autoloader, in a closed capsule holding a cassette of up to 12 autogrids, without exposure to atmosphere or open liquid nitrogen. This protects the lamellae from ice contamination and devitrification, which directly decides the yield and quality of the cryoET research. • R2 The new cryo-TEMs and cryo-PFIBs must use the same autogrid format as the six existing instruments, and the same cassette and capsule as the existing autoloader instruments, so that grids and lamellae can move between any old and new instrument, at either node, without re-clipping or re-mounting, and can be retrieved in the vitreous state. • R3 The new instruments must use the same TFS acquisition, automation and correlative software environment as the existing instruments (EPU, Tomography, MAPS correlative projects shared between TEM, SEM and FIB, and AutoTEM Cryo/WebUI for lamella preparation), so that targets, atlases, settings and trained procedures carry over between instruments and nodes. • R4 The AU node needs a cryo-PFIB for thick samples and tissues with four selectable ion species (Xe, Ar, O, N) switched in software, integrated cryo lift-out from autogrids and 3 and 6 mm high-pressure-freezing planchettes, and volume EM at cryo and room temperature. The existing gallium-ion Aquilos 2 is not well suited to thicker tissue-based samples. Published comparisons show that milling quality depends on the ion species used (Dumoux et al., eLife 2023) and besides Xenon and Argon, availability of Oxygen for biological samples is essential for the new workflows. • R5 Each node needs a 120 kV screening TEM that runs the same EPU, Tomography 5 and Maps 3 software as the Krios 5, so that screening results carry directly into high-end data collection.

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    From the notice

    • The purchase is a package of six electron microscopes from Thermo Fisher Scientific (TFS) for the Danish National Cryogenic Electron Microscopy Infrastructure (DANCEM): two Krios 5 300 kV cryo-TEMs (one at AU, one at UCPH), two Talos 12 120 kV TEMs (one at each node), one Arctis cryo-plasma-FIB (AU) and one Hydra Bio multi-ion cryo-plasma-FIB (AU). Two separate contracts will be issued, one for Aarhus University and one Copenhagen University concerning the relevant microscopes mentioned above.

    • Contact

      Ann Sophie Steffensenasst@au.dk
    • Type
      Result · Prequalification
    • Location
      Østjylland, Byen København
    • Main code
      Laboratory, optical and precision equipments (excl. glasses)
      38000000
    • 38511200Transmission electron microscope

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