1105601 UiO Livsvitenskap brukerutstyr - K930.22 MST instrument

Statsbygg bygger det nye Livsvitenskapsbygget (LVB) for Universitetet i Oslo og Oslo universitetssykehus på oppdrag fra Kunnskapsdepartementet og Helse Sør-Øst som medoppdragsgiver. Bygget skal være arbeidsplass for om lag 1.600 ansatte og 1.600 studenter. Oppstart for grave- og grunnarbeidene var 2. kvartal 2019 og planlagt ferdigstillelse er ultimo 2026. Her skal ulike fagmiljøer jobbe tverrfaglig for å utvikle nye løsninger på store utfordringer innen helse og bærekraft med tilgang til det beste og mest moderne utstyret som kreves for å drive forskning, undervisning og innovasjon i verdensklasse.Statsbygg intends to sign a contract with NanoTemper Technologies for the acquisition of a MST instrument, intrinsic tryptophan fluorescence, for the Life Sciences Building.We intend to purchase an MST instrument that is a capillary-based instrument for the analysis of molecular interactions using a combination of microscale thermophoresis (MST) and spectral shift with fluorescently labeled molecules as the principle of detection. The instrument must be an all-in-one, table-top model, and not exceed w400 x d600 x h550 mm in size due to space restrictions. The instrument must be able to measure both modalities in the same experiment on the same sample.We are researching, among other things, protein-protein and protein-ligand interactions, and the ability to analyze such interactions at equilibrium in a flow-free environment that does not require protein immobilization is a key feature that the equipment must satisfy. It is also essential that the system can measure many samples in series, in solution and with minimal use of material per sample. Through professional expertise in the laboratory, as well as research done online, we find that Monolith X is the only system with the following characteristics:Can do a combination of microscale thermophoresis (MST) and spectral shift with fluorescently labeled molecules as the principle of detection in the same instrument.Is an instrument that is an all-in-one, table-top model, not exceeding the size limitation.The Instrument can measure real-time changes in fluorescence intensity upon binding of ligand to the labeled protein while simultaneously changing the temperature of the sample (modality: microscale thermophoresis, MST).The instrument can measure real-time changes in fluorescence emission spectrum upon binding of ligand to the labeled protein as a result of a change in the fluorophore's emission spectrum (modality: spectral shift).The instrument can measure both modalities in the same experiment on the same sample.Can measure samples in very small volumes, using very small amounts of samples. See Appendix 4.0 Requirements MST Instrument K930.22 specification for more information on absolute requirements.Send an e-mail to kristine.milner.carlsen@statsbygg.no to receive the 4.0 Requirements MST Instrument K930.22 document. Summary:Given our technical requirements and needs, including in the attached requirement specification, the information we have and the investigations that have been carried out, to the best of our knowledge only Nanotemper technologies meets the requirements and can therefore, for technical reasons, deliver the service. The conditions in FOA § 13-4 (b) are deemed to be met. Any objections must be reported via e-mail to kristine.milner.carlsen@statsbygg.no within 10 days of the published announcement.